rabbit polyclonal anti scavenger receptor class b type i  (Novus Biologicals)

Journal: Journal of Lipid Research

Article Title: HDL inhibits the effects of oxidized phospholipids on endothelial cell gene expression via multiple mechanisms

doi: 10.1194/jlr.m047738

Figure Lengend Snippet: Fig. 2. The effect of HDL on OxPAPC signaling is not mediated by SR-BI. HAECs were transfected with either siRNA targeting SR-BI or scramble con- trol siRNA and treated 48 h later with media, Ox- PAPC (50 g/ml), HDL (50 g/ml), or OxPAPC and HDL (50 g/ml) as described in the Materials and Methods. The mRNA expression levels of KLF2 , ATF3 , INSIG1 , IL8 , and HO1 were determined by qPCR and normalized to GAPDH expression levels. All data are presented as average log2-fold change of triplicates ± SEM. Asterisks indicate signifi cance level in unpaired Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001) A: Despite effective silencing of SR-BI, the expression patterns of KLF2 , ATF3 , LDLR , and IL-8 after OxPAPC or OxPAPC+HDL treatment were unchanged. Results were confi rmed using two separate siRNAs. B: Similarly, blocking of SR-BI us- ing SR-BI antiserum (1:300 dilution) had no effect on the HDL response of KLF2 , ATF3 , LDLR , or IL-8 . Rabbit IgG was used as control. C: HAECs were ei- ther untreated or treated with OxPAPC (50 g/ml) in the presence or absence of HDL (50 g/ml) or delipidated apoA1 (25–50 g/ml). HDL and apoA1 were added for 1 h of pretreatment and 4 h of cotreatment. The mRNA expression levels of KLF2 , ATF3 , INSIG1 , IL-8 , and HO1 were determined by qPCR and normalized to GAPDH expression levels.

Article Snippet: Rabbit polyclonal antibodies targeting scavenger receptor class B type I (SR-BI) were purchased from Novus Biologicals (NB400-104 and NB400-134).

Techniques: Transfection, Expressing, Blocking Assay, Control